Identification of epitope recognized by a monoclonal antibody against VP2 protein of bluetongue virus serotype 8.

Mingxin Zang; Jiaxuan LI; Shuangyu Xie; Wen Cui; Yanping Jiang; Yigang XU; Xinyuan Qiao; Li Wang; Han Zhou; Min Liu; Yijing LI; Lijie Tang

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Identification of epitope recognized by a monoclonal antibody against VP2 protein of bluetongue virus serotype 8.

Author: Mingxin ZANG ¹ ; Jiaxuan LI ¹ ; Shuangyu XIE ¹ ; Wen CUI ¹ ; Yanping JIANG ¹ ; Yigang XU ¹ ; Xinyuan QIAO ¹ ; Li WANG ¹ ; Han ZHOU ¹ ; Min LIU ¹ ; Yijing LI ¹ ; Lijie TANG ¹
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1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
Publication Type:Journal Article
Keywords: VP2 protein; antigen epitope; bluetongue virus serotype 8; monoclonal antibody
From: Chinese Journal of Biotechnology 2017;33(8):1244-1252
CountryChina
Language:Chinese
Abstract: To confirm the B cell epitope recognized by monoclonal antibody (MAb) 3G11 of bluetongue virus type 8 (BTV-8) VP2 protein prepared in our laboratory, antigen epitopes recognized by 3G11 were screened and identified by phage display technology. KLLAT sequence was found by sequencing of blue spot after four rounds panning and 283LL284 of common short peptide sequence was obtained after comparison to amino acid sequence of BTV-8 VP2 protein. The peptide sequences KLLAA, KALAT, KLAAT and KLLAT were synthesized and identified by indirect ELISA. KLLAA and KLLAT bound strongly with supernatant and as cites of 3G11 cells and reacted specifically with BTV-8 positive standard sera. Further sequence analysis showed that amino acid sequence 283LL284 was conserved among different serotypes of BTV-8 strains, and283LL284 was the key amino acids of antigen epitopes recognized by 3G11. This study laid the foundation to establish type 8 BTV specific immunological detection methods.