Establishment of a high sensitive indirect ELISA for detecting specific antibodies against H9 subtype avian influenza virus.

Wei Zhang; Lidan Hou; Jie Song; Shuang Zhang; Yun LI; Jing LI; Lei Sun; Wenhui Fan; Wenjun Liu

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Establishment of a high sensitive indirect ELISA for detecting specific antibodies against H9 subtype avian influenza virus.

Author: Wei ZHANG ¹ ; Lidan HOU ² ; Jie SONG ¹ ; Shuang ZHANG ¹ ; Yun LI ¹ ; Jing LI ¹ ; Lei SUN ¹ ; Wenhui FAN ¹ ; Wenjun LIU ¹
Author Information

1. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
2. China Institute of Veterinary Drug Control, Beijing 100081, China.
Publication Type:Journal Article
Keywords: H9 subtype; avian influenza virus; hemagglutination inhibition test; hemagglutinin; indirect ELISA
From: Chinese Journal of Biotechnology 2017;33(8):1253-1264
CountryChina
Language:Chinese
Abstract: H9 subtype avian influenza virus causes worldwide epidemic, resulting in enormous economic losses of poultry production. In the present study, an indirect ELISA method was established for more accurate and specific detection. The recombinant protein of the globular head domain of HA of H9 subtype avian influenza virus was used as antigen. Specific blocking buffers and dilution buffers were determined to increase the sensitivity and specificity. The sensitivity of ELISA was higher than that of hemagglutination inhibition (HI) test. The coating antigen is very specific and no cross-reactivity with positive serum against H3N2, H5N2 and H7N9 subtype influenza viruses, Newcastle disease virus, avian infectious bronchitis virus, avian infectious disease virus, and egg drop syndrome virus. Two hundred of clinical sera samples were examined. The results indicate the coincidence rate between ELISA and HI test reached 97%. In addition, there was a positive correlation between OD450 values and the logarithm of HI titer to the base 2 of an individual serum sample (R2=0.981 1).