Effect of calcium on sporulation of Taiwanofungus camphoratus in submerged fermentation.
- Author:
Huaxiang LI
1
;
Zhenming LU
1
;
Qing ZHU
1
;
Yan GENG
1
;
Jinsong SHI
1
;
Zhenghong XU
1
;
Yanhe MA
2
Author Information
- Publication Type:Journal Article
- Keywords: Taiwanofungus camphoratus; calcium ion; calmodulin; conidiation; molecular mechanism
- From: Chinese Journal of Biotechnology 2017;33(7):1124-1135
- CountryChina
- Language:Chinese
- Abstract: Taiwanofungus camphoratus is a valuable and rare medicinal mushroom with various bioactivities, such as liver protection and anti-cancer. T. camphoratus can produce many arthroconidia at the end of submerged fermentation, but molecular mechanism underlying this submerged conidiation remains unknown. In this study, we found that Ca²⁺ concentration in culture medium significantly affected the arthroconidium production of T. camphoratus. Then, we identified two proteins (CaM and HSP90) involved in Ca²⁺/calmodulin signaling pathway and one protein (AbaA) involved in FluG-mediated conidiation pathway by two-dimensional electrophoresis analyses. Furthermore, we proposed a Ca²⁺/calmodulin- and FluG-mediated signaling pathway by bioinformatics analysis. By real-time quantitative PCR analyses of 23 key genes in the Ca²⁺/calmodulin- and FluG-mediated conidiation pathway, we found that expression levels of 7 genes (crz1, hsp90, flbB, brlA, abaA, wetA and fadA) showed significant responses to Ca²⁺ concentration in fermentation medium. Our research is beneficial for elucidating the underlying mechanism of submerged fermentation conidiation for T. camphoratus.
