Changes of urinary proteins in a bacterial meningitis rat model.

Author: Yanying NI ¹ ; Fanshuang ZHANG ¹ ; Manxia AN ¹ ; Youhe GAO ²
Author Information

1. Department of Pathophysiology, School of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China.
2. Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing 100875, China.
Publication Type:Journal Article
Keywords: LC-MS/MS; animal model; bacterial meningitis; early diagnose; urine proteome
From: Chinese Journal of Biotechnology 2017;33(7):1145-1157
CountryChina
Language:Chinese
Abstract: Unlike cerebrospinal fluid or blood, urine accumulates metabolic changes of the body and has the potential to be a promising source of early biomarkers discovery. Bacterial meningitis is a major cause of illness among neonates and children worldwide. In this study, we used Escherichia coli-injected rat model to mimic meningitis and collected urine samples on day 1 and day 3. We used two different methods to digest proteins and analyzed peptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We identified 17 and 20 differential proteins by two methods respectively on day 1, and 5 differential proteins by filter-aided digestion method on day 3. Finding these differential proteins laid a foundation to further explore biomarkers of bacterial meningitis.