Rapid cryopreservation for Siraitia grosvenorii cells based on cells' capacitance detection.

Jiarui LI; Zejian Wang; Meijin Guo; Yuanxin Guo; Shuai Huang; Yunfei Song; Zhen Sun; Yangyang Sun; Fanjing Kong; Yingping Zhuang

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Rapid cryopreservation for Siraitia grosvenorii cells based on cells' capacitance detection.

Author: Jiarui LI ¹ ; Zejian WANG ¹ ; Meijin GUO ¹ ; Yuanxin GUO ¹ ; Shuai HUANG ² ; Yunfei SONG ² ; Zhen SUN ¹ ; Yangyang SUN ¹ ; Fanjing KONG ¹ ; Yingping ZHUANG ¹
Author Information

1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
2. Guilin Layn Biotechnology Company, Guilin 541199, Guangxi, China.
Publication Type:Journal Article
Keywords: Siraitia grosvenorii cells; cells’ capacitance; cryopreservation; cryoprotectant; viable cell mass monitor
From: Chinese Journal of Biotechnology 2017;33(5):817-827
CountryChina
Language:Chinese
Abstract: A rapid quantitative evaluation method for Siraitia grosvenorii cells was successfully developed based on plant cells' capacitance value detected by a viable cell mass monitor and the cryopreservation of S. grosvenorii suspension cells was optimized. The survival rate of S. grosvenorii cells was quantitatively measured by viable cell mass monitor and 2, 3, 5-triphenyltetrazolium chloride (TTC). An optimum cryoprotectant recipe is that the growth medium contained 10% sucrose and 10% DMSO. The experimental results also showed higher cell survival rates and cell viabilities were achieved when suspension cells were treated with pretreatment of 0.2 mol/L sucrose. With the increase of concentration of sucrose, however, the cell survival rate was decreased. And the cell survival rate represented a bell shape with the increase of pretreatment time. The highest cell survival rate and cell viability were obtained with the 9 h' s pretreatment. In addition, there was a good correlation between the cell survival rate measured by cell recovery test and that measured by viable cell mass monitor, while there were no significant differences in the cell morphology and the ability of mogrosides V production by S. grosvenorii cells cultured in suspension after cryopreservation. Therefore, the evaluation method developed based on the viable cell mass monitor has good feasibility and reliability.