Detection of COL1A1/PDGFB fusion transcripts in dermatofibroscoma protuberans by revers transcriptase-polymerase chain reaction using paraffin-embedded tissues.
- Author:
Jiang-hua YANG
1
;
Wen-hao HU
;
Feng LI
;
Tian-cai LU
;
Hong-an LI
;
Bin CHANG
;
Xue-bao ZHANG
;
Xiao-ming ZHOU
;
Hong-wei DANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Child; Collagen Type I; genetics; Dermatofibrosarcoma; genetics; Female; Genes, sis; Humans; Male; Middle Aged; Paraffin Embedding; RNA, Messenger; analysis; Recombinant Fusion Proteins; genetics; Reverse Transcriptase Polymerase Chain Reaction; methods; Skin Neoplasms; genetics
- From: Chinese Journal of Pathology 2003;32(5):409-412
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the COL1A1/PDGFB fusion transcripts and discuss its clinicopathological significance in dermatofibroscoma protuberans.
METHODSFormalin fixed, paraffin-embedded tumor specimens from 12 patients with DFSP were reviewed by light microscope and the expression of COL1A1/PDGFB mRNA resulting from the reciprocal translocation t(17;22) (q22;q13.1) was detected by one-step revers transcriptase-polymerase chain reaction. The following tumor specimens were included as controls: 2 fibrosarcoma, 2 malignant fibrous histocytoma, 3 leiomyosarcoma, 1 dermarofibroma and 1 nerve shealth tumor.
RESULTSThe COL1A1/PDGFB fusion transcripts were detected in 8 (67%) of 12 samples from patients with DFSP. Nucleotide sequence analysis using the PCR products confirmed that different regions of the COL1A1 gene, respectively, were fused with of PDGFB gene. No COL1A1/PDGFB fusion transcripts were detected in the control tumors.
CONCLUSIONDetection of specific COL1A1/PDGFB fusion transcripts in DFSP will help to diagnose the nature of DFSP and research the mechanism of its molecular histogenesis.
