Study on the diagnostic significance of detecting the expression of AChR-gamma mRNA in rhabdomyosarcoma tissues.
- Author:
Bin CHANG
1
;
Feng LI
;
Li-juan PANG
;
Wen-hao HU
;
Tian-cai LU
;
Hong-an LI
;
Jian WANG
;
Xiong-zeng ZHU
;
Da-ren SHI
Author Information
- Publication Type:Journal Article
- MeSH: Diagnosis, Differential; Humans; Protein Subunits; RNA, Messenger; analysis; Receptors, Cholinergic; genetics; Receptors, Nicotinic; genetics; Reverse Transcriptase Polymerase Chain Reaction; Rhabdomyosarcoma; diagnosis
- From: Chinese Journal of Pathology 2003;32(5):413-416
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect over-expression of AChR-gamma mRNA in rhabdomyosarcoma tissues by duplex RT-PCR and discuss its potential in diagnosis of rhabdomyosarcoma.
METHODSDuplex RT-PCR was applied to the simultaneous detection of AChR-alpha and gamma subunit messenger RNA in 17 cases of rhabdomyosarcoma (9 ERMS, 6 ARMS, 2 PRMS). 20 cases of non-rhabdomyosarcomous small round cell tumors (6 poorly differentiated synovial sarcomas, 6 ES/PNET, 6 lymphomas, 2 neuroblastomas) and three normal muscle samples were also detected for AChR-alpha and gamma mRNA by the same method.
RESULTSAChR-alpha and AChR-gamma mRNA were expressed in all the cases of rhabdomyosarcoma. The rate of quantity in both transcripts was AChR-gamma/AChR-alpha >or= 1, but the rate for three normal muscle samples was < 1. Cases of non-rhabdomyosarcomous small round cell tumors were all negative for AChR-gamma.
CONCLUSIONAChR-gamma mRNA expression detected by molecular genetic methods is useful in diagnosis and differential diagnosis of rhabdomyosarcoma.
