Expression of monocyte chemoattractant protein-1 in experimental rat glomerulonephritis is mediated by NF-kappaB/IkappaB signal pathway.
- Author:
Ai-hua ZHANG
1
;
Song-ming HUANG
;
Gui-xia DING
;
Yuan-jun WU
;
Wei-zhen ZHANG
;
Hong-mei WU
;
Li FEI
;
Mei GUO
;
Rong-hua CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Chemokine CCL2; genetics; metabolism; Glomerulonephritis; chemically induced; genetics; metabolism; I-kappa B Proteins; metabolism; Kidney Glomerulus; metabolism; pathology; Kidney Tubules; metabolism; pathology; Male; NF-kappa B; metabolism; RNA, Messenger; genetics; metabolism; Rats; Rats, Sprague-Dawley; Signal Transduction
- From: Chinese Journal of Pathology 2003;32(6):548-552
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of NF-kappaB/IkappaB signal pathway in mediating the expression of monocyte chemoattractant protein-1 (MCP-1) in experimental rat glomerulonephritis.
METHODSNephrotoxic serum nephritis (NTN) was induced by injection of anti-GBM antibody into the tail veins of rats. Electrophoretic mobility shift assay (EMSA) and Western Blot were used to detect the activation of NF-kappaB, nuclear translocation of p65 subunit and degradation of IkappaBalpha and IkappaBbeta in rat renal tissue. MCP-1 expression in glomeruli and renal tubules was also assessed by immunohistochemistry and ribonuclease protection assay. This was further correlated with the activation of NF-kappaB.
RESULTSThere was an obvious expression of MCP-1 in glomeruli and renal tubules. Significant up-regulation of NF-kappaB activation, nuclear translocation of p65 subunit, and degradation of IkappaBalpha and IkappaBbeta were also observed in NTN rat renal tissue, as compared to the control group. A positive correlation was noted between NF-kappaB activation and MCP-1 expression.
CONCLUSIONSNF-kappaB/IkappaB signal pathway may play an important pathogenetic role in glomerulonephritis, with mediating the expression of MCP-1.
