- VernacularTitle:免疫相关性全血细胞减少症患者外周血淋巴细胞端粒长度研究
- Author:
Jiangbo ZHANG
1
;
Rong FU
1
;
Yihao WANG
1
;
Lijuan LI
1
;
Hui LIU
1
;
Kai DING
1
;
Chunyan LIU
1
;
Tian ZHANG
1
;
Shaoxue DING
1
;
Erbao RUAN
1
;
Wen QU
1
;
Huaquan WANG
1
;
Xiaoming WANG
1
;
Guojin WANG
1
;
Yuhong WU
1
;
Jia SONG
1
;
Hong LIU
1
;
Limin XING
1
;
Jing GUAN
1
;
Zonghong SHAO
1
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; B-Lymphocyte Subsets; immunology; Child; Female; Humans; Lymphocytes; ultrastructure; Male; Middle Aged; Pancytopenia; immunology; pathology; T-Lymphocyte Subsets; immunology; Telomere; ultrastructure; Young Adult
- From: Chinese Journal of Hematology 2014;35(7):605-608
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes of relative telomere length (RTL) of peripheral blood (PB) CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺T lymphocytes, CD19⁺B lymphocytes and bone marrow (BM) CD34⁺ cells and its association with disease severity in untreated patients with immuno-related pancytopenia (IRP).
METHODSThe PB CD3⁺ , CD3⁺ CD4⁺ , CD3⁺ CD8⁺ T lymphocytes, CD19⁺ B lymphocytes, and BM CD34⁺ cells were purified by magnetic activated cell sorting (MACS), and RTL were measured with flow-fluorescence in situ hybridization (FLOW-FISH).
RESULTSThe RTL of CD3⁺, CD3⁺CD4⁺ , and CD3⁺CD8⁺T lymphocytes in untreated IRP patients were (27.754 ± 16.323)%, (7.526 ± 3.745)% and (25.854 ± 14.789)%, respectivly, which were significantly shorter than those in healthy-controls (54.555 ± 19.782)%, (12.096 ± 2.805)%, and (38.367 ± 4.626)% (P<0.05). The RTL of CD19⁺ lymphocytes in untreated IRP patients was (22.136 ± 16.142)%, which was significantly shorter than that in healthy controls (42.846 ± 16.353)% (P<0.01). There was no significant difference of BM CD34⁺ cells RTL between the untreated IRP patients (22.528 ± 21.601)% and the healthy controls (23.936 ± 19.822)% (P>0.05). There were significantly positive correlations between the RTL of B lymphocytes and the count of white blood cell (r=0.706, P=0.015). There were negative correlations between RTL of B lymphocytes and the clinical symptoms (r=-0.613, P=0.045) and positive correlations with therapeutic effect (r=0.775, P=0.005).
CONCLUSIONThe shorter RTL of CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺, CD19⁺ lymphocytes, and the normal RTL of BM CD34⁺ cells in untreated IRP patients were identified, which might imply that IRP is a type of acquired autoimmune diseases.