Expression and clinical significance of miR-23a and metastasis suppressor 1 in colon carcinoma.
- Author:
Hai-lin TANG
1
;
Min DENG
;
Qian-jin LIAO
;
Xi ZENG
;
Xiu-tian ZHOU
;
Qi SU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Biomarkers, Tumor; metabolism; Colonic Neoplasms; metabolism; pathology; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; In Situ Hybridization; Lymphatic Metastasis; Male; MicroRNAs; metabolism; Microfilament Proteins; metabolism; Middle Aged; Neoplasm Invasiveness; Neoplasm Proteins; metabolism; Neoplasm Staging
- From: Chinese Journal of Pathology 2012;41(1):28-32
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of miR-23a and metastasis suppressor 1 (MTSS1) and their clinical significance in colon carcinoma.
METHODSA total of 92 cases of colon carcinomas were collected with both the tumor and paired normal tissue samples for the study. The miR-23a targeting MTSS1 was evaluated by luciferase reporter vector. Cell invasion potential was evaluated by trans-well invasion assay. In-situ hybridization and immunohistochemistry were used to detect miR-23a and MTSS1 expression.
RESULTSMiR-23a downregulated the expression of MTSS protein and enhanced the invasiveness of colon carcinoma. The expression rates of miR-23a and MTSS1 were 87.0% (80/92) and 17.4% (16/92) in colon carcinoma cases, respectively (P < 0.01). The up-regulation of miR-23a expression was associated with an advanced clinical stage (P = 0.029) and depth of invasion (P = 0.000). The expression of miR-23a was higher in the tumors with lymph node metastasis than those without (P = 0.041). Down-regulation of MTSS1 expression was associated with an advanced clinical stage (P = 0.027) and depth of invasion (P = 0.017). The expression of MTSS1 was lower in the tumors with lymph node metastasis than those without (P = 0.009). The expression of miR-23a had significantly negative correlation with that of MTSS1 (r = -0.594, P = 0.013).
CONCLUSIONSMiR-23a expression promotes colon carcinoma cell growth, invasion and metastasis through inhibition of MTSS gene. Both the low expression of MTSS1 and high expression of miR-23a may serve as important biological markers for the malignant phenotypes of colon cancer, such as invasion and metastasis.
