Influence of epidermal growth factor receptor monoclony antibody MAb225 on DNA repair of tongue carcinoma cell after radiation.

Author: Mingguo WANG ¹ ; Zhonghe WANG
Author Information

1. Dept. of Stomatology, The Affiliated Jinan Central Hospital of Shandong University, Jinan 250013, China.
Publication Type:Journal Article
MeSH: Antibodies, Monoclonal; Antigens, Nuclear; DNA Repair; DNA-Binding Proteins; Humans; Ku Autoantigen; Receptor, Epidermal Growth Factor; Tongue Neoplasms
From: West China Journal of Stomatology 2011;29(6):580-583
CountryChina
Language:Chinese
Abstract:
OBJECTIVEThe objective of this study is to investigate the effect of epidermal growth factor receptor (EGFR) monoclonal antibody MAb225 on repair of DNA double strand break (DNA-DSB) after radiation in tongue squamous cell carcinoma cell.
METHODSThe single cell gel electrophoresis (SCGE) was performed to estimate the repair of DNA-DSB induced by radiation in human tongue carcinoma cells Tca8113 treated with or without MAb225. Expression of Ku70 and Ku80 were detected by semiquantitative reverse transcription polymerase chain reaction and Western bolt.
RESULTSComet tail moment of MAb225 treated cell was significantly higher than untreated cell (P < 0.05). The expression of Ku70 and Ku80 were inhibited by MAb225.
CONCLUSIONMAb225 can inhibit repair of DNA-DSB induced by down-regulated expression of Ku70 and Ku80.