The effect of basic fibroblast growth factor on the gene expression of syndecan-4 by human periodontal ligament cell in culture.
- Author:
Jiaqing YAN
1
;
Chongtao LIN
;
Yuqin SHEN
;
Yan LIU
;
Xiaomin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Fibroblast Growth Factor 2; Humans; Periodontal Ligament; RNA, Messenger; Syndecan-4
- From: West China Journal of Stomatology 2011;29(6):588-591
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of basic fibroblast growth factor (bFGF) on the gene expression of syndecan-4 by human periodontal ligament cell (PDLC) in culture, and discuss the effect of bFGF on human PDLC proliferation and migration.
METHODS68 adolescent (12-18 years old) health premolar were collected, which were extracted for orthodontic reason. Human PDLC were cultured and stimulated by exogenous bFGF. After cultured 24, 48, 72h, gene expression of syndecan-4 was detected by SYBR green quantitative real time polymerase chain reaction.
RESULTSThe mRNA expression of syndecan-4 in 24 h group increased markedly than that in control group (P < 0.01), expecially in 1.0 ng x mL(-1) group. 1.0 ng x mL(-1) group in 48 h higher than that control group (P < 0.05). 1.0 ng x mL(-1) group in 72h compared with control group was lower (P < 0.05).
CONCLUSIONThe mRNA expression of syndecan-4 was increased by bFGF at the beginning, but the expression was decreased with the time. The expression of such changes may be one of the important factors which participate in the migration process of PDLC.
