Natural killer and cytotoxic T lymphocyte-mediated cytotoxicity enhanced by genetic overexpression of MHC class I chain-related protein A in oral squamous cell carcinoma: an experimental study in vivo.
- Author:
Chao LI
1
;
Fangqiong SHI
;
Dan YANG
;
Jie WANG
;
Xinchun JIAN
;
Canhua JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Squamous Cell; Cell Line, Tumor; Cytotoxicity, Immunologic; Genetic Therapy; Histocompatibility Antigens Class I; Humans; Killer Cells, Natural; Mice; Mice, Nude; Mouth Neoplasms; NK Cell Lectin-Like Receptor Subfamily K; Staphylococcal Protein A; T-Lymphocytes, Cytotoxic; Transfection
- From: West China Journal of Stomatology 2012;30(1):32-35
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect on natural killer (NK) and cytotoxic T lymphocyte (CTL)-mediated cytotoxicity by genetic overexpression of MHC class I chain-related protein A (MICA) in oral squamous cell carcinoma (OSCC).
METHODSThe OSCC cells by genetic overexpression of MICA were detected to identify the biological features including cell growth curve, cell cycle distribution, plate clone forming rate and tumorigenicity in nude mice. The expression of natural killer group 2, member D (NKG2D) receptor and the cytotoxicity to target tumor cells of NK92 and CTL cells, which co-cultured with the transfected OSCC cells or the non-transfected or blank vector-transfected controls, were measured by flow cytometry and lactate dehydrogenase (LDH) release assay.
RESULTSThere was no difference in biological features before and after MICA gene transfection to OSCC cells. Flow cytometry and LDH release assay showed that MICA-overexpressed OSCC cells enhanced the cytotoxicity to target tumor cells and up-regulated the expression of NKG2D on NK92 and CTL (P<0.05).
CONCLUSIONMICA may be considered as a promising immunotherapy target of OSCC.
