The construction and expression of Saccharomyces albicans pACT1-GFP plasmids.
- Author:
Jing SUN
1
;
Fen JIA
;
Ming-Hui XIA
;
Hua QIAN
;
Hongnan DONG
;
Qingguo QI
Author Information
- Publication Type:Journal Article
- MeSH: Candida albicans; Green Fluorescent Proteins; Humans; Plasmids; Saccharomyces; Saccharomyces cerevisiae
- From: West China Journal of Stomatology 2012;30(2):115-118
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct strains containing green fluorescent protein (GFP) to study gene regulation in Saccharomyces albicans cells during the infection process.
METHODSpACT1-GFP was constructed, and Saccharomyces albicans CAI4 was transformed. The expression of GFP in yeast and hyphal compartments was observed with microscopy.
RESULTS99% of Saccharomyces albicans cells containing pACT1-GFP fusion displayed significant fluorescence levels both in the yeast and hyphal compartments. The fluorescence intensity in two compartments had no obvious difference.
CONCLUSIONpACT1-GFP can be expressed stably in the yeast cells.
