The Protein Expressions of Apoptosis-associated Genes in the Obstructed Ureters of Rats.
10.4111/kju.2006.47.2.189
- Author:
Hyeong Gon KIM
1
;
Sung Hyun PAICK
;
Yong Soo LHO
;
Hyun Hoe KIM
;
Chul KWAK
Author Information
1. Department of Urology, Konkuk University College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Ureteral obstruction;
Apoptosis;
Rats
- MeSH:
Animals;
Apoptosis;
bcl-X Protein;
Blotting, Western;
Ligation;
Prostaglandin-Endoperoxide Synthases;
Rats*;
Rats, Sprague-Dawley;
Ureter*;
Ureteral Obstruction
- From:Korean Journal of Urology
2006;47(2):189-194
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The role of apoptosis in the pathogenesis of ureteral damage from obstructive uropathy has rarely been studied. This study was performed to determine the protein expression of the apoptosis-associated genes in the pathogenesis of ureteral damage during the course of obstructive uropathy in ligated rat ureters. MATERIALS AND METHODS: After unilateral ligation of the ureter, each group of five Sprague-Dawley rats was sacrificed and examined at 1, 5, 10, 15, 20, 25, 30 and 35 days after ligation: five rats with normal ureters were also examined as controls. The protein expressions of the fas-associated death domain (FADD), Bax, Bcl-xL and cyclooxygenases (COX)-2 genes in obstructed ureters were assessed by performing Western blotting. RESULTS: The expressions of FADD protein in the 20 and 25 day-obstructed ureters (DOUs) were significantly higher than that in control ureters and the peak was reached in the 25 DOUs. The expressions of Bcl-xL protein in the 20, 25 and 30 DOUs were significantly higher than that in the control ureters and the peak was reached in the 25 DOUs. The expression of COX-2 protein in the 5, 10, 15, 25 DOUs were significantly higher than that in the control ureters and the peak was reached in the 10 DOUs. CONCLUSIONS: The FADD and Bcl-xL genes were involved in apoptosis of the obstructed ureter. The peaks of their expressions were at 25 DOUs. The expression of the COX-2 gene may be related with apoptosis in the obstructed ureter.
