Expression of P38 MAPK and MMP-2 mRNA in neonatal rats with hyperoxia-induced lung injury.
- Author:
Tao PAN
1
;
Zhi-Hui XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Female; Hyperoxia; complications; Lung; pathology; Lung Injury; metabolism; Male; Matrix Metalloproteinase 2; genetics; RNA, Messenger; analysis; Rats; Rats, Sprague-Dawley; Tissue Inhibitor of Metalloproteinases; genetics; p38 Mitogen-Activated Protein Kinases; metabolism
- From: Chinese Journal of Contemporary Pediatrics 2013;15(5):383-386
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe P38 mitogen-activated protein kinase (P38 MAPK) and matrix metalloproteinase-2 (MMP-2) mRNA expression level changes in neonatal rats with hyperoxia-induced lung injury,and to investigate the influence of P38 MAPK activation on MMP-2 mRNA expression.
METHODSThirty-six Sprague-Dawley (SD) rats were randomly divided into three groups: air control, hyperoxia and SB203580-treated hyperoxia (n=12). The rats were sacrificed on the 3rd and 7th days and the lungs were removed. Hematoxylin-eosine staining was used to observe the pathological changes in lung tissues.
RESULTSCompared with the air and SB203580-treated groups, levels of P38 MAPK and MMP-2 mRNA significantly increased in the hyperoxia group (P<0.01).
CONCLUSIONSExpression of P38 MAPK increases in neonatal rats with hyperoxia-induced acute lung injury and this may play a role in control of the expression of MMP-2 mRNA.
