Effects of umbilical cord blood monocytes transplantation on EPO protein and oligodendrocyte progenitors in neonatal rats with hypoxic-ischemic brain damage.
- Author:
Jia-Fen JI
1
;
Jin-Ping ZHANG
;
Xiao-Li WANG
;
Qing-Jie MU
;
Meng-Meng FAN
;
Yu-Xi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Erythropoietin; analysis; biosynthesis; Fetal Blood; cytology; Hypoxia-Ischemia, Brain; metabolism; pathology; therapy; Monocytes; transplantation; Oligodendroglia; pathology; Rats; Rats, Sprague-Dawley; Stem Cells; pathology
- From: Chinese Journal of Contemporary Pediatrics 2013;15(9):775-778
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of umbilical cord blood monocytes (UCBMC) transplantation on erythropoietin (EPO) protein and oligodendrocyte progenitor cells in hypoxia-ischemia (HI) neonatal rats.
METHODSForty seven-day-old Sprague-Dawley rats were randomly divided into normal control (N), HI, UCBMC and HI+UCBMC groups (n=10 each). Hypoxic-ischemic brain damage (HIBD) model was prepared according to the Rice method. Twenty-four hours after hypoxia, the N and HI groups were injected with 2 μL phosphate buffered saline (PBS), and the UCBMC and HI+UCBMC groups were injected with 3×10(6) UCBMC via the lateral ventricle. EPO protein and oligodendrocyte progenitor cells in the subventricular zone of the injured brain were observed by EPO/DAPI and NG2/DAPI immunofluorescence double staining, and their correlation was analyzed.
RESULTSSeven days after transplantation, there were more NG2(+)DAPI(+) and EPO(+)DAPI(+) cells in the HI+UCBMC group than in the UCBMC (P<0.05), N and HI groups (P<0.01). More NG2(+)DAPI(+) and EPO(+)DAPI(+) cells were observed in the UCBMC group compared with the N and HI groups (P<0.01). There were more NG2(+)DAPI(+) cells in the N group than in the HI group (P<0.01). The number of NG2(+)DAPI(+) cells was correlated with the number of EPO(+)DAPI(+) cells in the HI+UCBMC group (r=0.898, β=1.4604, P<0.01).
CONCLUSIONSUCBMC can promote expression of oligodendrocyte progenitor cells, which is correlated with an increase in EPO protein and thus repairs brain white matter damage in neonatal rats with HIBD.