Effect of crocin on intracellular calcium concentration in cultured bovine aortic smooth muscle cells.
- Author:
Shu-ying HE
1
;
Zhi-yu QIAN
;
Fu-tian TANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta, Thoracic; Calcium; metabolism; Carotenoids; isolation & purification; pharmacology; Cattle; Cells, Cultured; Chloroform; antagonists & inhibitors; Gardenia; chemistry; Hydrogen Peroxide; antagonists & inhibitors; Intracellular Space; metabolism; Muscle, Smooth, Vascular; cytology; metabolism; Myocytes, Smooth Muscle; metabolism; Plants, Medicinal; chemistry
- From: Acta Pharmaceutica Sinica 2004;39(10):778-781
- CountryChina
- Language:English
-
Abstract:
AIMTo study the effect of crocin on intracellular calcium concentration ([Ca2+]i) in cultured bovine aortic smooth muscle cells (BASMCs).
METHODSCells were loaded with fluorescence probe Fluo-3/AM and [Ca2+]i was measured by laser scanning confocal microscope (LSCM).
RESULTSIn the presence or absence of extracellular Ca2+, crocin (1 x 10(-8), 1 x 10(-7), 1 x 10(-6) mol x L(-1)) concentration-dependently inhibited the [Ca2+]i elevation induced by 1 x 10(-2) mol x L(-1) H2O2 (for the former, the inhibition rates were 34.1%, 57.1% and 74.3%, while for the latter were 26.2%, 32.1%, 50.0%). In the absence of extracellular Ca2+, crocin (1 x 10(-8), 1 x 10(-7), 1 x 10(-6) mol x L(-1)) could inhibit the [Ca2+]i elevation induced by 70 mmol x L(-1) CHCl3, the inhibition rates were 27.8%, 27.8% and 50.0% respectively.
CONCLUSIONCrocin could inhibit the extracellular Ca2+ influx and release of intracellular Ca2+ stores in endoplasmic reticulum.
