Influence of N,N'-diacetyl-L-cystine on D-galactosamine/lipopolysaccharide induced immunological liver failure in mice.
- Author:
Pei WANG
1
;
Xing-chang REN
;
Jin YU
;
Yi LIN
;
Xi-ming WU
Author Information
- Publication Type:Journal Article
- MeSH: Alanine Transaminase; blood; Animals; Aspartate Aminotransferases; blood; Bilirubin; blood; CD4 Antigens; metabolism; CD8 Antigens; metabolism; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cystine; analogs & derivatives; pharmacology; Liver; pathology; Liver Failure, Acute; blood; pathology; Male; Mice; Mice, Inbred BALB C; T-Lymphocytes; pathology
- From: Acta Pharmaceutica Sinica 2004;39(10):782-786
- CountryChina
- Language:English
-
Abstract:
AIMTo study the therapeutic effects of N,N'-diacetyl-L-cystine (DiNAC) on immunological liver failure.
METHODSSerum ALT, AST and T cell subsets in peripheral blood of the experimental animals during the trial period were analyzed by an automatic serum analyzer and a flow cytometer, respectively. The sectioned liver specimens were examined under a light microscope. And 24 h after the injection of Gal/LPS, the survival rate of rats was calculated.
RESULTSDiNAC (50, 200, 800 mg x kg(-1), i.p.) suppressed the elevation of serum levels of ALT and AST, markedly enhanced proliferation and differentiation of T cell subsets (CD4+, CD8+ and Th1, Th2), and improved all the histopathological features. In mice of fulminant hepatic failure (FHF), the survival time significantly prolonged and the survival rate increased 24 h after i.p. DiNAC. These effects were obviously dose-dependent.
CONCLUSIONDiNAC on mice with FHF has an inhibitory action which is related to immune mechanism.
