Absorption of zedoary oil in rat intestine using in situ single pass perfusion model.
- Author:
Jian YOU
1
;
Qing-po LI
;
Ying-wei YU
;
Fu-de CUI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biological Transport; Colon; metabolism; Curcuma; chemistry; Duodenum; metabolism; Ileum; metabolism; In Vitro Techniques; Intestinal Absorption; Jejunum; metabolism; Male; Perfusion; Plant Oils; chemistry; isolation & purification; pharmacokinetics; Plants, Medicinal; chemistry; Rats; Rats, Wistar; Sesquiterpenes; isolation & purification; pharmacokinetics; Sesquiterpenes, Germacrane; isolation & purification; pharmacokinetics
- From: Acta Pharmaceutica Sinica 2004;39(10):849-853
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the absorption of zedoary oil in intestine of rat.
METHODSIn situ single pass perfusion model was used and the concentrations of three components in perfusate were determined by HPLC in combination with diode array detection.
RESULTSThe P(app) s of curcumol, curdione and germacrone were all low and had no significant difference (P > 0.05) at zedoary oil concentration of 0.4, 0.8 and 1.2 mg x mL(-1) in transmucosal fluid or in four different regions of intestine of rat [duodenum, jejunum, ileum, colon]. The absorption rates of germacrone and curdione were faster than curcumol's in this study.
CONCLUSIONThe zedoary oil concentration in transmucosal fluid had no significant effect on the P(app) s within the scope of 0.4-1.2 mg x mL(-1). The absorption of curcumol, curdione and germacrone showed the passive diffusion process, and didn't contain a special absorption window.
