Effects of isoliensinine on proliferation of porcine coronary arterial smooth muscle cells induced by phenylephrine.
- Author:
Jun-hua XIAO
1
;
Yan-lin ZHANG
;
Li-li DING
;
Xiu-ling FENG
;
Jia-ling WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Cells, Cultured; Coronary Vessels; cytology; Dose-Response Relationship, Drug; Fibroblast Growth Factor 2; metabolism; HSP70 Heat-Shock Proteins; metabolism; Isoquinolines; administration & dosage; isolation & purification; pharmacology; Muscle, Smooth, Vascular; cytology; Nelumbo; chemistry; Phenylephrine; antagonists & inhibitors; Plants, Medicinal; chemistry; Proto-Oncogene Proteins c-fos; metabolism; Proto-Oncogene Proteins c-myc; metabolism; Proto-Oncogene Proteins c-sis; metabolism; Swine
- From: Acta Pharmaceutica Sinica 2005;40(2):105-110
- CountryChina
- Language:English
-
Abstract:
AIMTo investigate the inhibitory effects and mechanism of action of isoliensinine (IL) on the proliferation of porcine coronary arterial smooth muscle cells (CASMCs) induced by phenylephrine (Phen) and its mechanisms of action.
METHODSMTT assay, immunohistochemical method and Western blotting were adopted.
RESULTSIL (0.03 - 3 micromol x L(-1)) could inhibit the CASMCs proliferation induced by Phen (0.1 micromol x L(-1)) in a concentration-dependent manner. IL (0.1 micromol x L(-1)) antagonized Phen-induced overexpression of PDGF-beta and bFGF from 0.545 +/- 0.026 and 0.47 +/- 0.03 to 0.458 +/- 0.019 and 0.376 +/- 0.017 (P < 0.01 , P < 0.01). IL (0.1 micromol x L(-1)) also decreased c-fos, c-myc and hsp70 overexpression induced by Phen from 0.57 +/- 0.04, 0.44 +/- 0.04 and (173 +/- 36)% to 0.46 +/- 0.05, 0.372 +/- 0.021 and (115 +/- 35)% respectively (P < 0.01, P < 0.01, P < 0.01).
CONCLUSIONIL exerted antiproliferative effect on CASMCs induced by phenylephrine, and its mechanisms were related to decrease the overexpression of growth factors (PDGF-beta, bFGF), protooncogene (c-fos, c-myc) and hsp70.
