5-HT2 receptor mediated the potentiation of GABA-activated current in the membrane of the dorsal root ganglion neurons of rat.
- Author:
Jie AN
1
;
Chang-Hua CHEN
;
Bing-cai GUAN
;
Ming TANG
;
Cheng-gao YU
;
Zhi-wang LI
Author Information
- Publication Type:Journal Article
- MeSH: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; pharmacology; Animals; Cyproheptadine; pharmacology; Female; Ganglia, Spinal; cytology; physiology; Male; Membrane Potentials; drug effects; Neurons; physiology; Patch-Clamp Techniques; Protein Kinase C; antagonists & inhibitors; Rats; Rats, Sprague-Dawley; Receptors, Serotonin, 5-HT2; Serotonin; analogs & derivatives; pharmacology; Serotonin 5-HT2 Receptor Agonists; Serotonin 5-HT2 Receptor Antagonists; Signal Transduction; gamma-Aminobutyric Acid; pharmacology
- From: Acta Pharmaceutica Sinica 2005;40(1):1-7
- CountryChina
- Language:English
-
Abstract:
AIMTo explore the modulation of 5-HT on GABA-activated current (I(GABA)) in the membrane of rat dorsal root ganglion (DRG) neurons and its mechanism.
METHODSRat DRG neurons were isolated mechanically and enzymatically, on which whole-cell patch clamp recording and repatch technique for intracellular dialysis were performed.
RESULTSIn the majority of neurons examined (92.0%, 69/75) GABA induced a concentration-dependent inward current. In neurons sensitive to GABA preapplication of 5-HT produced potentiation effect (82.6% , 57/69) on I(GABA). Preapplication of 5-HT at concentrations of 1 x 10(-6), 1 x 10(-5), 1 x 10(-4) and 1 x 10(-3) mol x L(-1) potentiated I(GABA) by (35 +/- 8)% (n=8), (47 +/- 11)% (n=10), (65 +/- 17)% (n=9) and (75 +/- 18)% (n=11), respectively. This effect was mimicked by alpha-methyl-5-HT (1 x 10(-6) mol x L(-1)), a specific 5-HT2 receptor agonist, and reversed by cyproheptadine, a selective 5-HT2 receptor antagonist. The potentiation of I(GABA) by 5-HT was irrespective to whether the I(5-HT) presents or not in a subset of neurons. The concentration-response curves for GABA before and after pretreatment with 5-HT manifested the same threshold value and similar EC50 (2.0 x 10(-5) and 1.9 x 10(-5) mol x L(-1), respectively) , while the maximal value of I(GABA) for the latter was 33.6% higher than that for the former. Intracellular dialysis with GDP-beta-S or H-7 abolished the potentiation of I(GABA) by 5-HT, while H-9 did not.
CONCLUSION5-HT can potentiate GABA-activated current via PKC-dependent phosphorylation of GABA(A) receptor following the activation of 5-HT2 receptor.
