Liquid chromatography frontal analysis of the protein binding of glimepiride.
- Author:
Da-wei ZHOU
1
;
Huai-feng WANG
;
Fa-mei LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Humans; Hypoglycemic Agents; metabolism; Protein Binding; Serum Albumin; metabolism; Sulfonylurea Compounds; metabolism
- From: Acta Pharmaceutica Sinica 2005;40(1):39-42
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the protein binding of glimepiride.
METHODSAn HPLC-FA method is performed by using Pinkerton GFF II-S5-80 internal-surface reversed-phase silica support (150 mm x 4.6 mm ID, 5 microm) at pH 7.4 in a 67 mmol x L(-1) isotonic sodium phosphate buffer at 37 degree C. Other conditions included flow rate of 0.2 mL x min(-1), UV detection at wavelength 230 nm and injection volume 900 microL.
RESULTSNonlinear regression parameter estimation was used for the association constant measurement of glimepiride to both primary and secondary sites, which were 5.1 (micromol x L(-1)-1 and 1 for K1 and n1, and 0.017 (micromol x L(-1))-1 and 7 for K2 and n2, respectively.
CONCLUSIONThe method is shown to be suitable for investigation of protein binding of glimepiride.
