SHENG MAI ZHUSHEYE improves the viability and movement parameters of human sperm in vitro.
- Author:
Fang ZHANG
1
;
Ji-Xiu XU
;
Hua-Gang MA
;
Lan-Ying ZHOU
;
Zhen-Lian CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Survival; drug effects; Drugs, Chinese Herbal; pharmacology; Humans; In Vitro Techniques; Male; Sperm Motility; drug effects; Spermatozoa; drug effects
- From: National Journal of Andrology 2009;15(5):468-471
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of SHENG MAI ZHUSHEYE on the movement parameters and viability of human sperm in vitro.
METHODSWe collected sperm samples from 33 normal fertile men, divided each into two, and cultured them in vitro with SHENG MAI ZHUSHEYE + Hams-F10 and Hams-F10 alone, respectively. Then we measured the straight line velocity (VSL), curvilinear velocity (VCL), average path velocity (VAP) and the amplitude of lateral head displacement (ALH) of the sperm by computer-aided semen analysis at 0.5, 1, 2, 4, 8 and 12 h. And the sperm viability was detected.
RESULTSVCL was significantly higher at 8 h (P < 0.05) and very significantly higher at 12 h (P < 0.01) in the SHENG MAI ZHUSHEYE + Hams-F10 group than in the Hams-F10 group. VSL, VAP and ALH were significantly increased in the former group at 4, 8 and 12 h as compared with the latter (P < 0.05). The sperm viability was significantly decreased in the Hams-F10 group at 12 h (P < 0.05).
CONCLUSIONSHENG MAI ZHUSHEYE can improve sperm movement parameters and increase sperm viability in vitro.
