Expression of the voltage-dependent anion channel gene in human ejaculated spermatozoa.
- Author:
Xiang XU
1
;
Yong WANG
;
Zhou YU
;
Jing CHEN
;
Meng GUO
;
Yao-ting GUI
;
Zhi-ming CAI
Author Information
- Publication Type:Journal Article
- MeSH: Comparative Genomic Hybridization; Gene Expression; Humans; Infertility, Male; genetics; metabolism; Male; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Reverse Transcriptase Polymerase Chain Reaction; Sperm Motility; Spermatozoa; metabolism; Voltage-Dependent Anion Channel 2; genetics
- From: National Journal of Andrology 2009;15(6):493-498
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the genes involved in sperm motility.
METHODSWe hybridized asthenospermia and normal motile sperm cDNA samples with the human whole genome Affymetrix chip to screen differentially expressed genes. Then we detected the mRNA expressions of the voltage-dependent anion channel genes (VDACs) in human organs and spermatozoa by RT-PCR and compared their expressions in the poor and normal motility spermatozoa.
RESULTSDifferentially expressed genes VDACs were identified by analysis of the hybridization signals, including the 3 subtypes VDAC1, VDAC2 and VDAC3. The expression of VDAC2 mRNA was significantly decreased in the poor motility sperm (0.568 +/- 0.036), as compared with the healthy men (0.803 +/- 0.043, P < 0.01).
CONCLUSIONThe decreased expression of VDAC2 in the ejaculated spermatozoa is possibly associated with the reduction of sperm motility.
