Expression of high mobility group protein A in male mouse testicular cell lines.
- Author:
Zheng-bing MA
1
;
Ying YANG
;
Jun WANG
;
Lei LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Division; Cell Line; Cell Proliferation; Gene Expression; HMGA1a Protein; genetics; HMGA2 Protein; genetics; Male; Mice; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction; Spermatogenesis; Testis; cytology; metabolism
- From: National Journal of Andrology 2009;15(6):534-537
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression of high mobility group protein A (HMGA) in male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts), and to pave the theoretical ground for further investigation of the action mechanism of the HMGA gene in male mouse spermatogenesis.
METHODSWe detected the expressions of HMGA1 and HMGA2 by RT-PCR and Western blot in the male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts).
RESULTSHMGA1 and HMGA2 were expressed in the male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts) at both mRNA and protein levels. Western blot and RT-PCR methods showed similar results.
CONCLUSIONThe expression of HMGA may be involved in the cell division and proliferation of TM4, GC-1spg and GC-2spd(ts) and play an important role in spermatogenesis of male mice.