Nandeshi: a powerful inhibitor of human acrosin activity.
- Author:
Gui-Hong SONG
1
;
Jue ZHANG
;
Xiao-Meng ZHANG
;
Wei-Wei NING
;
Ya-Zhong JI
;
Ning HUI
;
Ya-Nan ZHAO
;
You-Jun ZHOU
;
Ju ZHU
;
Jia-Guo LÜ
Author Information
- Publication Type:Journal Article
- MeSH: Acrosin; antagonists & inhibitors; metabolism; Contraceptive Agents, Female; pharmacology; Enzyme Inhibitors; pharmacology; Humans; Male; Spermatozoa; drug effects; Tosyllysine Chloromethyl Ketone; pharmacology
- From: National Journal of Andrology 2009;15(8):700-702
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the inhibitory effect of Nandeshi, an acrosin inhibitor, on human acrosin activity.
METHODSWe collected sperm samples from 10 healthy fertile men and cultured them with Nandeshi at 30 degrees C for 5 minutes at the concentrations of 0. 100, 0.120, 0.144, 0.173, 0.207, 0.249, 0.299, 0.358 and 0.430 mmol/L, with the controls treated with a well-known acrosin inhibitor N-alpha-p-tosyl-L-lysine chloromethylketone (TLCK) at 150.0, 189.8, 213.6, 240.3, 270.3, 304.1 and 342.1 mmol/L. Then we determined the residual activity of human acrosin by improved Kennedy assay.
RESULTSThe residual activity of acrosin was negatively correlated with the Nandeshi concentration, and Nandeshi exhibited an inhibition rate about 800 times that of TLCK.
CONCLUSIONNandeshi has a powerful inhibitory effect on human acrosin, and improved Kennedy assay is a simple, practical and highly sensitive technique for the detection of human acrosin activity.
