Expressions of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines and their significance.
- Author:
Yong-guang JIANG
1
;
Jia-hui ZHAO
;
Yong LUO
;
Da-lin HE
;
Nan LI
;
Xin-hao CUI
;
Tao PENG
Author Information
- Publication Type:Journal Article
- MeSH: Cadherins; metabolism; Cell Line, Tumor; Humans; Male; Prostatic Neoplasms; metabolism; pathology; beta Catenin; metabolism
- From: National Journal of Andrology 2009;15(10):867-871
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expressions of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines and explore their relationship with the metastasis of human prostate cancer.
METHODSThe expressions and distribution of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines (IF11 and IA8) were detected by Western blot and immunofluorescent staining.
RESULTSThe expression of E-cadherin was high in LNCaP, but absent in IF11 and IA8, while beta-catenin was expressed highly in IF11 and LA8, but lowly in LNCaP. Immunofluorescent staining showed that E-cadherin was mainly in the membrane of LNCaP, while beta-catenin both in the membrane of LNCaP and in the nuclei of IF11 and IA8.
CONCLUSIONE-cadherin and beta-catenin are differently expressed and distributed in prostate cancer cell lines with different characteristics of epithelial-mesenchymal transition (EMT), and the abnormal activation of the beta-catenin signal pathway may be involved in the EMT of prostate cancer cells.
