Impact of adenovirus-mediated PDE5-shRNAs on the free calcium level in the smooth muscle cells of rat corpus cavernosum.
- Author:
Yun-gao PAN
1
;
Ji-hong LIU
;
Ying ZHAN
;
Tao WANG
;
Zhi-hua WAN
;
Zhong-yuan LI
;
Yun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Calcium; metabolism; Cyclic Nucleotide Phosphodiesterases, Type 5; metabolism; Erectile Dysfunction; therapy; Gene Silencing; Genetic Therapy; Male; Myocytes, Smooth Muscle; metabolism; Penis; cytology; RNA, Small Interfering; metabolism; Rats; Rats, Sprague-Dawley
- From: National Journal of Andrology 2009;15(10):882-885
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the influence of recombinant adenovirus-mediated PDE5-shRNAs on the free calcium level in rat penile smooth muscle cells and to explore the feasibility of gene therapy for erectile dysfunction (ED).
METHODSSmooth muscle cells of the rat corpus cavernosum were transfected with constructed rAd-rPDE5-shRNAs and then dyed with the calcium fluorescent probe Fluo-3/AM at 24, 48 and 72 hours. The dynamic changes of the calcium fluorescence intensity were observed under the laser scanning confocal microscope (LSCM). The relative level of intracellular calcium was determined by fluorescence indexes.
RESULTSThe fluorescence indexes of calcium at 24, 48 and 72 hours were 829.3 +/- 7.8, 801.5 +/- 9.5 and 856.3 +/- 8.7 in the rAd-rPDES-shRNAs group, significantly lower than in the rAd-mock (1106.3 +/- 10.8, 1121.3 +/- 10.2 and 1058.5 +/- 12.1) and blank control group (1076.6 +/- 9.7, 1133.4 +/- 11.2 and 1104.3 +/- 10.5) (P < 0.05).
CONCLUSIONAdenovirus mediated shRNAs of the target PDE5 gene can significantly decrease the intracellular calcium level in the smooth muscle cells of the rat corpus cavernosum.
