Identification of TGF-beta/Smads pathway in human prostate cancer cell lines and its significance.
- Author:
Kai-jie WU
1
;
Guang-feng ZHU
;
Dong ZHANG
;
Jin ZENG
;
Xin-yang WANG
;
Yan XUE
;
Lin-lin ZHANG
;
Da-lin HE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Humans; Male; Prostatic Neoplasms; metabolism; pathology; Signal Transduction; Smad2 Protein; metabolism; Transforming Growth Factor beta; metabolism
- From: National Journal of Andrology 2009;15(10):920-924
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the TGF-beta/Smads signal pathway in different human prostate cancer cell lines, and to explore the role of the TGF-beta/Smads pathway in the progression and metastasis of prostate cancer and its possible mechanisms.
METHODSWe detected the expressions of the key proteins involved in the TGF-beta/Smads pathway, TbetaR II, Smad2/3, p-Smad2 and Smad4, in prostate cancer cell lines LNCaP, PC-3, DU145, IF11 and IA8 with different metastatic potentials by Western blotting.
RESULTSTbetaR II was expressed highly in PC-3, DU145, IF11 and IA8, but extremely lowly in LNCaP. Smad2/3 was highly expressed in all the cell lines with different intensity, while p-Smad2 only in PC-3 and DU145. The expression of Smad4 was detected in LNCaP, PC-3 and DU145, but not in IF11 and IA8.
CONCLUSIONThe status of the TGF-beta/Smads pathway differs in the cell lines with different metastatic potentials, only active in PC-3 and DU145. The TGF-beta/Smads pathway may be involved in the invasion and metastasis of prostate cancer through altering the expressions of the key proteins in it.
