Microwave radiation decreases the expressions of occludin and JAM-1 in rats.
- Author:
Xiao-Fang GAO
1
;
Shui-Ming WANG
;
Rui-Yun PENG
;
Hong-Yan ZUO
;
Li-Feng WANG
;
Ya-Bing GAO
;
Ji DONG
;
Zhen-Tao SU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood-Testis Barrier; metabolism; radiation effects; Cell Adhesion Molecules; metabolism; Down-Regulation; Male; Membrane Proteins; metabolism; Microwaves; Occludin; Rats; Rats, Wistar; Testis; metabolism; radiation effects
- From: National Journal of Andrology 2010;16(1):10-13
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the changes in the expressions of the tight junction related protein occludin and junctional adhesion molecule-1 (JAM-1) of the blood-testis barrier and their significance in rats after microwave radiation.
METHODSEighty male Wistar rats were exposed to microwave radiation with average power density of 0, 10, 30 and 100 mW/cm2 for five minutes, and dynamic changes in the expressions of testicular occludin and JAM-1 were observed by Western blot and image analysis at 6 h, 1 d, 3 d, 7 d and 14 d after the radiation.
RESULTSThere was a significant down-regulation in the expression of the occludin protein at 3 - 7 d, 6 h - 7 d and 6 h - 14 d (P < 0. 05), as well as in that of JAM-1 at 3 - 7 d, 1 - 7 d and 1-14 d (P < 0.05) after exposure to 10, 30 and 100 mW/cm2 microwave radiation.
CONCLUSIONThe decreased protein expressions of occludin and JAM-1 may play an important role in the microwave radiation induced-damage to the blood-testis barrier.
