Surveillance program on and the distribution related to the virulence-associated genes of Vibrio cholerae in estuary of Pearl River
10.3760/cma.j.issn.0254-6450.2011.12.014
- VernacularTitle:珠江河口水体霍乱弧菌监测及菌株毒力基因分布特征
- Author:
Bai-Sheng LI
1
;
Duo-Chun WANG
;
Hai-Ling TAN
;
Bi-Xia KE
;
Jing-Diao CHEN
;
Dong-Mei HE
;
Mei-Zhen LIU
;
Xiao-Ling DENG
;
Chang-Wen KE
;
Biao KAN
Author Information
1. 广东省疾病预防控制中心
- Keywords:
Vibrio cholerae;
Virulence gene;
Pulsed field gel electrophoresis
- From:Chinese Journal of Epidemiology
2011;32(12):1242-1246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the distribution,molecular characteristics and virulence genes of the O1 and O139 Vibrio cholerae isolates from the Pearl River Estuary water.Methods Vibrio cholerae isolates collected from the Pearl River estuary waters from January 2009 to December 2010,were tested by PCR for eight virulence-related genes,including cholera toxin(ctxA),zonula occludens toxin(zot),accessory cholera enterotoxin(ace),hemolysin(hlyA),toxin-coregulated pilus (tcpA),outer membrane protein(ompU),and the regulatory protein genes(tcpⅠ,toxR).Genetic relation was assessed by pulsed-field gel electrophoresis(PFGE)and the patterns were clustered by BioNumerics.Results From 1152 aquatic samples,69 isolates were identified,including 41 Inaba,18 Ogawa and 10 O139.All the isolates showed ctxA negative,while the hlyA and toxR genes were positive in all the isolates.34.15％(14/41)of the Inaba strains were hlyA + toxR + ompU + ace + zot + tcpI+,while 66.67％(12/18)belonged to Ogawa strains and 70％(7/10)of the O139 strains were hlyA + toxR+.Through PFGE analysis,the O1 isolates formed three clusters in this study.The patterns of O1 isolates differed widely,with the similarity as 72.8％-100.0％,while the patterns of O139 isolates having the similarity of 69.9％-95.5％.Conclusion The non-toxigenic O1 and O139 V.cholerae had a wide distribution in the environment of Pearl River estuary water during the nonepidemic period of cholera.All the aquatic isolates presented diversities on the related virulent genes.
