A Preliminary Study on the Expression and Regulation of C-X-C Chemokine Genes in a Human Colon Epithelial Cell Line.
- Author:
Suk Kyun YANG
;
Ok Hee KIM
;
Me Hwa LEE
;
Seon Me PARK
;
Hwoon Yong JUNG
;
Weon Seon HONG
;
Young Il MIN
- Publication Type:Original Article
- Keywords:
Chemokine;
Epithelial cell;
Colon
- MeSH:
Bacterial Infections;
Chemokines;
Colon*;
Cytokines;
Epithelial Cells*;
Granulocyte-Macrophage Colony-Stimulating Factor;
Humans*;
Immune System;
Interleukin-1alpha;
Interleukin-6;
Interleukin-8;
Lymphocytes;
Neutrophils;
RNA;
RNA, Messenger;
Salmonella
- From:Journal of the Korean Society of Coloproctology
1998;14(1):21-26
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Human colon epithelial cells secrete an array of proinflammatory cytokines that includes IL-8, MCP-1, GM-CSF, TNF alpha and IL-6. This response may serve to attract neutrophils and macrophags to the site of infection. In addition to IL-8 and MCP-1, the chemokine family contains other members, which, alone or in combination, can recruit and/or activate inflammatory and lymphoid cells. In this study, we asked whether colon epithelial cells express a broader array of chemokines than previously described. The colon epithelial cell line, Caco-2, was stimulated for 3h with IL-1 alpha, or was infected with Salmonella dublin. RNA was extracted and chemokine mRNA levels were determined by quantitative reverse transcription-PCR using internal RNA standards. Ex pression of GRO alpha, GRO beta, GRO gamma and IP-10 increased by bacterial infection or IL-l alpha stimulation. These data strongly support the notion that epithelal cells are an important and integral component of the host's natural immune system.
