Screening 20 Chinese herbs often used for clearing heat and dissipating toxin with nude mice model of hepatitis C viral infection.
- Author:
Zhi-min TANG
1
;
Meng PENG
;
Chun-jiao ZHAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antiviral Agents; pharmacology; Drug Evaluation, Preclinical; Drugs, Chinese Herbal; pharmacology; Female; Gentiana; chemistry; Hepacivirus; drug effects; Hepatitis C; virology; Male; Mice; Mice, Nude; Phytotherapy; Scutellaria baicalensis; chemistry; Sophora; chemistry; Virus Replication; drug effects
- From: Chinese Journal of Integrated Traditional and Western Medicine 2003;23(6):447-448
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo seek effective drugs for anti-hepatitis C virus by screening 20 Chinese herbs often used for clearing heat and dissipating toxin with nude mice model of hepatitis C viral (HCV) infection.
METHODSAfter the model mice had been treated with selected drug for 3 months, transmission electron microscope was used to observe whether the HCV-like particles in human fetal hepatocytes (HFH) transplanted into mice spleen still existed, and quantitative RT-PRC technique was used to detect the serum content of HCV-RNA before and after treatment.
RESULTS(1)HCV-like particles existed in all the model mice after treatment. (2) Serum content of HCV-RNA decreased after treated with Radix Gentianae, Radix Scutellariae, Radix Sophorae tonkinensis, Fructus Gardeniae and Fructus Sophorae flavoscentis, but unchanged after treatment with other drugs.
CONCLUSIONAll the 20 herbs screened has not effect in directly eradicating HCV, but Radix Gentianae, Radix Scutellariae, Radix Sophorae tonkinensis, Fructus Gardeniae and Fructus Sophorae flavoscentis could significantly inhibit the replication of HCV-RNA.
