Effect of chailing decoction and its active ingredients on experimental nephritis in rats.
- Author:
Ping LI
1
;
Pei-heng LI
;
Zi-yi ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; Drugs, Chinese Herbal; chemistry; pharmacology; Female; Nephritis; chemically induced; pathology; Oleanolic Acid; analogs & derivatives; pharmacology; Proteinuria; pathology; Rats; Rats, Wistar; Saponins; pharmacology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2003;23(10):757-759
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen the active ingredients of Chailing decoction (CLD) by using rat nephritis model induced by mono-colonal antibody 1-22-3 (mAb) injection.
METHODSThe active ingredients of CLD was screened by 5 successive times of experiment. In each time, 28 rats were randomly divided into 4 groups, 7 in each. Group 1 was treated with PBS as control, Groups 2-4 were treated separately with CLD and its various ingredients, the medication was started 5 days before and lasted to 8 days after modeling by peritoneal injection, 13 days totally. All the rats were killed 8 days after modeling to observe the effect of various drugs on proteinuria, morphological change of kidney and biochemical parameters.
RESULTSCLD, Xiaochaihu decoction, various combination of thorowax root and its extract (saikosaponin-d) could reduce urinary protein, inhibit the proliferation of mesangial cell and expansion of extracellular matrix.
CONCLUSIONCLD and its active ingredients had inhibition on mAb induced rat model of nephritis, the active is saikosaponin-d.
