Effect of wenxin capsule on myocyte apoptosis and expression of Bcl-2, Fas gene protein in rats of experimental myocardial ischemia.
- Author:
Teng ZHANG
1
;
Hong-xin CAO
;
Xiao-yu SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Drug Combinations; Drugs, Chinese Herbal; pharmacology; Isoproterenol; Male; Myocardial Ischemia; chemically induced; metabolism; pathology; Myocytes, Cardiac; pathology; Proto-Oncogene Proteins c-bcl-2; biosynthesis; genetics; Rats; Rats, Wistar; fas Receptor; biosynthesis; genetics
- From: Chinese Journal of Integrated Traditional and Western Medicine 2003;23(10):769-771
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the molecular mechanism of Wenxin Capsule (WXC, an effective Chinese composite drug) in preventing and treating myocardial ischemia of coronary heart disease.
METHODSRat model of myocardial ischemia was established by subcutaneous multi-point injecting isoproterenol. Effect of WXC on cell apoptosis was observed by transmission electron microscopy and TUNEL method, and its effect on apoptotic related gene Bcl-2 and Fas gene protein expression was observed by immunohistochemical method.
RESULTSIsoproterenol induced myocardial ischemic injury could cause evident cardial cell apoptosis, obvious enhance Fas gene protein expression and mild enhance Bcl-2 gene protein expression. WXC could significantly down-regulate Fas, up-regulate Bcl-2 gene protein expression, significantly inhibit and block the myocardial cell apoptosis.
CONCLUSIONSTo inhibit and block the event of cell apoptosis through regulating Bcl-2 and Fas gene protein expression in ischemic myocardium might be one of the mechanisms of WXT in preventing and treating myocardial ischemic injury of coronary heart disease.
