Comparative study on the regulatory effects on senescence related cell cycle gene expression by TCM principles of tonifying shen, invigorating pi benefiting qi, and activating blood circulation.
- Author:
Fang-min CHEN
1
;
Wei-kang ZHAO
;
Pin-chu XU
Author Information
- Publication Type:Journal Article
- MeSH: Aging; genetics; Animals; Cell Cycle; drug effects; Cells, Cultured; Cellular Senescence; genetics; Cyclin D1; biosynthesis; genetics; Cyclin-Dependent Kinase Inhibitor p16; biosynthesis; genetics; Down-Regulation; Drugs, Chinese Herbal; pharmacology; Fibroblasts; cytology; Gene Expression Regulation; Lung; cytology; Male; Medicine, Chinese Traditional; Proliferating Cell Nuclear Antigen; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2003;23(11):837-840
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the effects of TCM therapeutic principles of tonifying Shen (TS), benefiting Qi (BQ), invigorating Pi (IP) and activating blood circulation (ABC) herbs in regulating the gene expression in senescence related cell cycle.
METHODSDrug sera containing TCM herbs of the above-mentioned principles were used to treat the aged human diploid fibroblast cell line 2BS. The effect of TCM on the senescence related cell cycle and its related gene expression (P16INK4, Cyclin D1 and PCNA) were examined by means of cell proliferative doublings, flow cytometry, RT-PCR and Western blot analysis.
RESULTSTCM herbs of TS and BQ could improve the cell cycle, down-regulate the P16 and Cyclin D1 mRNA/protein expression, up-regulate PCNA mRNA/protein expression, while TCM herbs of IP and ABC showed insignificant effect on these indexes.
CONCLUSIONTCM herbs of TS and BQ have effect in improving cell cycle, it may be achieved through promoting the P16 pathway of gene expression.
