Cloning and bioinformatic analysis of lovastatin biosynthesis regulatory gene lovE.
- Author:
Xin HUANG
1
;
Hao-ming LI
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Aspergillus; genetics; metabolism; Cloning, Molecular; Fungal Proteins; chemistry; genetics; metabolism; Hydrophobic and Hydrophilic Interactions; Lovastatin; biosynthesis; genetics; Molecular Sequence Data; Sequence Homology, Amino Acid
- From: Chinese Medical Journal 2009;122(15):1800-1805
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDLovastatin is an effective drug for treatment of hyperlipidemia. This study aimed to clone lovastatin biosynthesis regulatory gene lovE and analyze the structure and function of its encoding protein.
METHODSAccording to the lovastatin synthase gene sequence from genebank, primers were designed to amplify and clone the lovastatin biosynthesis regulatory gene lovE from Aspergillus terrus genomic DNA. Bioinformatic analysis of lovE and its encoding animo acid sequence was performed through internet resources and software like DNAMAN.
RESULTSTarget fragment lovE, almost 1500 bp in length, was amplified from Aspergillus terrus genomic DNA and the secondary and three-dimensional structures of LovE protein were predicted.
CONCLUSIONIn the lovastatin biosynthesis process lovE is a regulatory gene and LovE protein is a GAL4-like transcriptional factor.
