Acute lung injury induced by H9N2 virus in mice.

Yan LI; Yunfeng Shan; Ying Chi; Tian Wen; Xiaodong Han

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Acute lung injury induced by H9N2 virus in mice.

Author: Yan LI ¹ ; Yunfeng SHAN ² ; Ying CHI ² ; Tian WEN ² ; Xiaodong HAN ³
Author Information

1. Department of Histology, Medical School, Nanjing University, Nanjing, Jiangsu 210093, China.
2. Institute of Pathogenic Microbiology, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu 210009, China.
3. Department of Histology, Medical School, Nanjing University, Nanjing, Jiangsu 210093, China. Emait: hanxd@nju. edu. cn.
Publication Type:Journal Article
MeSH: Acute Lung Injury; blood; etiology; virology; Animals; Bronchoalveolar Lavage Fluid; chemistry; Female; Influenza A Virus, H9N2 Subtype; pathogenicity; Interleukin-1; blood; metabolism; Interleukin-6; blood; metabolism; Mice; Mice, Inbred C57BL; Respiratory System; virology; Tumor Necrosis Factor-alpha; blood; metabolism
From: Chinese Medical Journal 2014;127(20):3576-3580
CountryChina
Language:English
Abstract:
BACKGROUNDH9N2 avian influenza viruses (AIVs) have repeatedly caused infections in mammals even humans in many countries. The purpose of our study was to evaluate the acute lung injury (ALI) caused by H9N2 viral infection in mice.
METHODSSix- to eight- week-old female SPF C57BL/6 mice were infected intranasally with 1 × 10(4) MID50 of A/HONG KONG/2108/2003 [H9N2 (HK)] virus. Clinical signs, pathological changes, virus titration in tissues of mice, arterial blood gas, and cytokines in bronchoalveolar lavage fluid (BALF) and serum were observed at different time points after AIV infection.
RESULTSH9N2-AIV-infected mice exhibited severe respiratory syndrome, with a mortality rate of 50%. Lung histopathological changes in infected mice included diffuse pneumonia, alveolar damage, inflammatory cellular infiltration, interstitial and alveolar edema, and hemorrhage. In addition, H9N2 viral infection resulted in severe progressive hypoxemia, lymphopenia, and a significant increase in interleukin 1, interleukin 6, tumor necrosis factor, and interferon in BALF and serum.
CONCLUSIONSThe results suggest that H9N2 viral infection induces a typical ALI in mice that resembles the common features of ALI. Our data may facilitate the future studies of potential avian H9N2 disease in humans.