Th and Treg response induced by Aspergillus fumigatus pulsed dendritic cells in vitro.

Runchao Wang; Zhe Wan; Ruoyu LI

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Th and Treg response induced by Aspergillus fumigatus pulsed dendritic cells in vitro.

Author: Runchao WANG ¹ ; Zhe WAN ¹ ; Ruoyu LI ²
Author Information

1. Department of Dermatology, Research Center for Medical Mycology, Peking University First Hospital, Peking University, Beijing 100034, China.
2. Department of Dermatology, Research Center for Medical Mycology, Peking University First Hospital, Peking University, Beijing 100034, China. Email: mycolab@126.com.
Publication Type:Journal Article
MeSH: Animals; Aspergillus fumigatus; pathogenicity; Cytokines; metabolism; Dendritic Cells; immunology; microbiology; Forkhead Transcription Factors; metabolism; Interleukin-12; metabolism; Male; Mice; Mice, Inbred C57BL; T-Lymphocytes, Helper-Inducer; immunology; T-Lymphocytes, Regulatory; immunology; Th1 Cells; immunology; Transforming Growth Factor beta; metabolism
From: Chinese Medical Journal 2014;127(20):3616-3622
CountryChina
Language:English
Abstract:
BACKGROUNDDendritic cells (DCs) can recognize the pathogen-associated molecular patterns (PAMP) of Aspergillus fumigatus (A. fumigatus), activating the immune response. During A. fumigatus infection, a Th and Treg response induced in the fungi-pulsed DCs is not yet well understood.
METHODSIn this study, bone marrow-derived dendritic cells (BMDCs) were separated and proliferated from C57BL/6 mice. A. fumigatus pulsed DCs were generated and cultured with CD4(+) T cells derived from the spleen of C57BL/6 mice in vitro. CD4(+) T cells differentiation after co-culture were analyzed by flow cytometry, ELISA, and real-time PCR analysis.
RESULTSThe A. fumigatus pulsed DCs exhibited increased Th1 and Treg frequency, Th1-related cytokines (IFN-γ and IL-12), Treg-related cytokines (TGF-β) and T-bet, and Foxp3 mRNA levels compared with the control group. There was no significant difference between A. fumigatus pulsed DCs group and the control group about Th17 and Th2 frequency.
CONCLUSIONSThe inactivated conidia of A. fumigatus were able to activate BMDCs and made them capable of triggering T cell responses in vitro. A. fumigatus loaded DCs was a weak inducer of Th17 and Th2, but induced a strong Th1 and Treg response.