Oenanthe javanica extract increases immunoreactivities of antioxidant enzymes in the rat kidney.

Hyun-jin Tae; Joon Ha Park; Jeong-hwi Cho; In Hye Kim; Ji Hyeon Ahn; Jae Chul Lee; Jong-dai Kim; Jinseu Park; Soo Young Choi; Moo-ho Won

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Oenanthe javanica extract increases immunoreactivities of antioxidant enzymes in the rat kidney.

Author: Hyun-Jin TAE ¹ ; Joon Ha PARK ² ; Jeong-Hwi CHO ² ; In Hye KIM ² ; Ji Hyeon AHN ² ; Jae Chul LEE ² ; Jong-Dai KIM ³ ; Jinseu PARK ¹ ; Soo Young CHOI ¹ ; Moo-Ho WON ⁴
Author Information

1. Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 200-702, South Korea.
2. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
3. Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, South Korea.
4. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. Email: mhwon@kangwon.ac.kr.
Publication Type:Journal Article
MeSH: Animals; Antioxidants; metabolism; Catalase; metabolism; Glutathione Peroxidase; metabolism; Kidney; drug effects; enzymology; metabolism; Male; Oenanthe; chemistry; Oxidative Stress; drug effects; Plant Extracts; chemistry; pharmacology; Rats; Rats, Sprague-Dawley; Superoxide Dismutase; metabolism
From: Chinese Medical Journal 2014;127(21):3758-3763
CountryChina
Language:English
Abstract:
BACKGROUNDOenanthe javanica is an aquatic perennial herb originated from East Asia. Nowadays, the effects of Oenanthe javanica have been proven in various disease models. Studies regarding the antioxidant effect of Oenanthe javanica in the kidney are still unclear.
METHODSThis study was therefore performed to investigate the effect of the Oenanthe javanica extract (OJE) in the rat kidney using immunohistochemistry for antioxidant enzymes, copper, zinc-superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT) and glutathione peroxidase (GPx). Sprague-Dawley rats were randomly assigned to three groups: (1) normal diet fed-group (normal-group), (2) diet containing ascorbic acid (AA)-fed group (AA-group) as a positive control, (3) diet containing OJE-fed group (OJE-group). AA and OJE were supplied during 28 days.
RESULTSThe side-effects were not observed in all the groups. Immunoreactivities of SOD1, SOD2, CAT and GPx were easily detected in the distal tubules of the kidney, and their immunoreactivities in the AA-and OJE-groups were increased to about 1.4-1.5 times and 2 times, respectively, compared with those in the normal-group.
CONCLUSIONOJE significantly increased expressions of SOD1 & 2, CAT and GPx immunoreactivities in the distal tubules of the rat kidney, and this finding suggests that significant enhancements of endogenous enzymatic antioxidants by OJE treatment may be a legitimate strategy for decreasing oxidative stresses in the kidney.