Development and application of morphological analysis method in Aspergillus niger fermentation.
- Author:
Wenjun TANG
;
Jianye XIA
;
Ju CHU
;
Yingping ZHUANG
;
Siliang ZHANG
- Publication Type:Journal Article
- MeSH:
Aspergillus niger;
cytology;
Fermentation;
Industrial Microbiology;
Reproducibility of Results
- From:
Chinese Journal of Biotechnology
2015;31(2):291-299
- CountryChina
- Language:Chinese
-
Abstract:
Filamentous fungi are widely used in industrial fermentation. Particular fungal morphology acts as a critical index for a successful fermentation. To break the bottleneck of morphological analysis, we have developed a reliable method for fungal morphological analysis. By this method, we can prepare hundreds of pellet samples simultaneously and obtain quantitative morphological information at large scale quickly. This method can largely increase the accuracy and reliability of morphological analysis result. Based on that, the studies of Aspergillus niger morphology under different oxygen supply conditions and shear rate conditions were carried out. As a result, the morphological responding patterns of A. niger morphology to these conditions were quantitatively demonstrated, which laid a solid foundation for the further scale-up.
