Effect of oxygen-vectors on the production of ε-poly-L-lysine.
- Author:
Fangfang BO
;
Zhaoxian XU
;
Zhuzhen SUN
;
Changhong CAO
;
Jun XIA
;
Hong XUI
;
Xiaohai FENG
- Publication Type:Journal Article
- MeSH:
Alkanes;
chemistry;
Batch Cell Culture Techniques;
Bioreactors;
Fermentation;
Oxygen;
chemistry;
Polylysine;
biosynthesis
- From:
Chinese Journal of Biotechnology
2015;31(3):431-435
- CountryChina
- Language:Chinese
-
Abstract:
To enhance the production of ε-poly-L-lysine (ε-PL) by improving dissolved oxygen level of the fermentation system, different oxygen-vectors were added to broth and n-dodecane was screened as the best oxygen-vector. The best amount of n-dodecane was 0.5% (V/V) and the best time was at start of the fermentation. In a fed-batch fermentation in a 5 L bioreactor, ε-PL concentration reached a maximum of (30.8 ± 0.46) g/L and the dry cell weight obtained was (33.8 ± 0.29) g/L, increasing by 31.6% and 20.7% compared with the control group, respectively. This improvement can be related to 0.5% n-dodecane could maintain dissolved oxygen concentration > 32% of air concentration compared with 23.8% in ε-PL production phase, and the production of a main by-product, poly-L-diaminopropionic acid, fell by 31%. These results indicated that the dissolved oxygen level in the broth was improved by adding n-dodecane, which can inhibit the by-product production and improve the biosynthesis of ε-PL.
