Progress in co-immobilization of multiple enzymes.
- Author:
Jindan WANG
;
Guangya ZHANG
- Publication Type:Journal Article
- MeSH:
Biocatalysis;
Enzymes, Immobilized
- From:
Chinese Journal of Biotechnology
2015;31(4):469-480
- CountryChina
- Language:Chinese
-
Abstract:
Enzyme immobilization is the core technology of biocatalysis. Over the past few decades, enzyme immobilization research mainly focused on single enzyme immobilization. In recent years, multi-enzyme immobilization attracts more and more attention as it could increase the local concentration of reaction and improve the reaction yield. In this review, a summary of the recent progress, together with our research, is presented. Special emphasis is placed on four methods in multi-enzymes co-immobilization, namely, the nonspecific covalent co-immobilization, the nonspecific non-covalent co-immobilization, the non-covalent encapsulation co-immobilized and the site specificity co-immobilized. Finally, some industrial uses of immobilized multi-enzymes were addressed and the application prospect of multi-enzyme immobilization was highlighted.
