Optimization and application of targeted LC-MS metabolomic analyses in photosynthetic cyanobacteria.
- Author:
Xiangfeng NIU
;
Xiaoqing ZHANG
;
Xinheng YU
;
Yingxue SU
;
Lei CHEN
;
Weiwen ZHANG
- Publication Type:Journal Article
- MeSH:
Chromatography, Liquid;
Mass Spectrometry;
Metabolome;
Metabolomics;
Photosynthesis;
Synechocystis;
metabolism
- From:
Chinese Journal of Biotechnology
2015;31(4):577-590
- CountryChina
- Language:Chinese
-
Abstract:
To accurately analyze metabolites in industry-important photosynthetic microbes, LC-MS based metabolomics protocol needs to be optimized specifically for individual species. In this study, an LC-MS based metabolomics method was optimized for cyanobacterium Synechocystis sp. PCC 6803. With the optimized extraction, liquid chromatographic and mass spectral parameters, the method was capable of detecting 24 important metabolites related to central carbohydrate and energy metabolism in Synechocystis sp. PCC 6803. The study laid an important foundation for the metabolomics analysis of cyanobacteria.
