Improvement of natamycin production in an industrial strain by heterologous expression of the afsRS(cla) global regulatory genes.
- Author:
Zhengsheng TAO
;
Yemin WANG
;
Hualiang ZHENG
;
Meifeng TAO
- Publication Type:Journal Article
- MeSH:
Anti-Bacterial Agents;
biosynthesis;
Genes, Regulator;
Industrial Microbiology;
Multigene Family;
Natamycin;
biosynthesis;
Streptomyces;
genetics
- From:
Chinese Journal of Biotechnology
2015;31(5):744-751
- CountryChina
- Language:Chinese
-
Abstract:
The afsRS(cla) global regulatory genes from Streptomyces clavuligerus activate the production of two antibiotics in Streptomyces lividans. In this study, we gained an increase of 38% in the production of natamycin (3.56 g/L) in an industrial strain Streptomyces gilvosporeus TZ1401 through the integration of pHL851 that bears the afsRS(cla) global regulatory genes into its genome. We discovered by quantitive real-time reverse transcription PCR (qRT-PCR) that the expression of 6 genes of the natamycin biosynthetic gene cluster were improved from 1.9 to 2.7 times. This suggests that afsRS(cla) improve the production of natamycin through increased transcription. This study provides a good example for applying afsRS(cla) in high yield breeding of industrial antibiotic producers.
