Progress of new-generation genome editing mediated by engineered endonucleases.
- Author:
An XIAO
;
Bo ZHANG
- Publication Type:Journal Article
- MeSH:
Clustered Regularly Interspaced Short Palindromic Repeats;
Endonucleases;
genetics;
Genetic Engineering;
methods;
Genetic Therapy;
Genome;
Genomics
- From:
Chinese Journal of Biotechnology
2015;31(6):917-928
- CountryChina
- Language:Chinese
-
Abstract:
Genome editing refers to the experimental methods to targeted modify specific loci in the genomic DNA sequence. In recent years, engineered endonucleases, including ZFN, TALEN and CRISPR/Cas, have been developed as a new-generation genome editing technique, and greatly improved the feasibility of gene function analyses, gene therapy, etc. Here, we briefly summarize the basic principle, developmental process and applications of this technology.
