Relationship between interferon-α conformation and its anti-viral activity determined by circular dichroism and flow cytometry.
- Author:
Maozhi HU
;
Wenhua LI
;
Qiuxiang YAN
;
Yan YANG
;
Qing SUN
;
Zhiming PAN
;
Guiyou CUI
;
Xin'an JIAO
- Publication Type:Journal Article
- MeSH:
Antiviral Agents;
chemistry;
Circular Dichroism;
Flow Cytometry;
Humans;
Interferon-alpha;
chemistry;
Protein Structure, Secondary;
Recombinant Proteins;
chemistry
- From:
Chinese Journal of Biotechnology
2015;31(11):1651-1659
- CountryChina
- Language:Chinese
-
Abstract:
The relationship between the conformation of interferon-α (IFN-α) and its anti-viral activity were analyzed by circular dichroism (CD) and flow cytometry (FCM) techniques. The recombinant human IFN-α (rIFN-α2b and rIFN-α2a) were used. CD spectra from 190 nm to 240 nm indicated that two the IFN-α showed stable secondary structure at 65 degrees C, but unstable when the temperature was above 65 degrees C, and the change was irreversible. FCM data of the anti-viral activity of IFN-α indicated that the change of its secondary structures partly weakened its anti-viral activity. The rIFN-α2b and rIFN-α2a showed the same phenomenon. These data indicated that the conformation of IFN-α is one of the factors to influence its anti-viral activity and the combination of CD and FCM is a good method to analyze the relationship between the conformation of protein drugs and their biological activities in single cell level.
