Collagen quantitation by detection of marker peptides with HPLC-MS.
- Author:
Kun SUN
;
Fan YANG
;
Yingjun KONG
;
Jiyao KANG
;
Wei CAO
;
Xiaoyan YANG
;
Shenghua ZHA
;
Guifeng ZHANG
;
Minglin WANG
- Publication Type:Journal Article
- MeSH:
Animals;
Cattle;
Chromatography, High Pressure Liquid;
Collagen Type I;
analysis;
Mass Spectrometry;
Peptides;
analysis
- From:
Chinese Journal of Biotechnology
2015;31(11):1660-1668
- CountryChina
- Language:Chinese
-
Abstract:
A method for quantitation of collagen was established by detecting marker peptide with high performance liquid chromatography-mass spectrometry (HPLC-MS). Theoretical marker peptides were selected by sequence comparison. Bovine collagen type I was digested with trypsin. Marker peptides typical for collagen type I were identified with HPLC-MS. The relationship between the abundance of marker peptides and collagen concentration was established. The results show that GEAGPSGPAGPTGAR and the other 5 peptides showed high resolution during chromatographic separation and high signal intensity during MS analysis. Peptide signal intensity and collagen concentration showed a good linear relationship in the range from 0.1 to 3 mg/mL. Bovine tendon and collagen sponge were used as actual samples and collagen contents were determined as 90.2% and 93.4% respectively. Quantitation of marker peptides of collagen was a feasible method to identify and quantify collagens in medical device research and development.
