Susceptibility change to HBV in primary culture of first trimester human fetal hepatocytes.
- Author:
Bo TANG
1
;
Yu-ming WANG
;
Fang WANG
;
Jun LIU
;
Rui ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; drug effects; Cells, Cultured; Female; Fetus; virology; Hepatitis B virus; growth & development; Hepatocytes; cytology; physiology; virology; Humans; Phenobarbital; pharmacology; Pregnancy; Pregnancy Trimester, First
- From: Chinese Journal of Hepatology 2004;12(1):21-24
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESBy culturing primary early (8 to 12-weeks-old) human fetal hepatocytes with different conditions, to study the status of cell susceptibility to HBV.
METHODSDuring primary culture of 10-weeks-old human fetal hepatocytes with serum-free medium adding different differentiation-induced ingredients, to inoculate cell with HBV at certain time. Cell shape, function and markers of HBV infection are measured.
RESULTS6 days after seeding, markers of mature hepatocytes are observed in cells cultured with 2.5mmol/L phenobarbital sodium, and these cells show susceptibility to HBV. Other ingredients cannot render hepatocytes susceptible to HBV.
CONCLUSIONPhenobarbital sodium induces differentiation and susceptibility to HBV in primary culture of early human fetal hepatocytes.
